Fully-Chemically Synthesized Peptide with Activity
Equivalent to FGF2

PG-011 is composed of a cyclic peptide that has binding affinity for FGFR1c and exhibits agonist activity toward FGFR1c.

PG-011 exhibits binding affinity for FGFR1c and agonist activity.

*This is for illustrative purpose.

iPSCs Pluripotency Maintenance Ability

We evaluated the proliferation and pluripotency maintenance abilities of iPSCs using PG-011. The culture media were supplemented with either FGF2 (100 ng/mL, approx. 5.9 nM) or an equivalent molar concentration of PG-011 (approx. 30 ng/mL). After 7 days of culture, subculturing was performed. Following three rounds of subculturing, the morphology of the iPSCs was observed under a microscope. The results confirmed that iPSCs cultured with PG-011 exhibited a morphology similar to that of cells cultured with FGF2.

After the third round of subculturing, iPSCs were collected on day 7 of culture, and the percentage of positive cells for pluripotency markers (SSEA4, TRA-1-60) was measured using flow cytometry to assess the pluripotent state of the iPSCs. The results confirmed that, for all three cell lines evaluated, PG-011 and FGF2 showed similar rates of positive pluripotency markers. This confirms that PG-011 has equivalent iPSC pluripotency maintenance capability to FGF2.

Superior Stability

We compared the stability of PG-011 and FGF2. Each sample was incubated at 37°C in serum-free medium for 1 day and 4 days. After incubation, the samples were evaluated for their proliferation activity on MSCs (concentration condition: 0.06 nM). The results showed that while FGF2 exhibited a decrease in activity over the incubation time, PG-011 maintained its activity even after 4 days of incubation. This indicates that PG-011 possesses superior stability compared to FGF2.

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